1. A spectrophotometer measures light given off by a substance light changed (change in wavelength) by a substance light that passes through a substance light that is fluoresced by a substance
2. Ultraviolet light has a short wavelength is measured at 185 to 300 nm is harmful to human eyes all of the above
3. Beer's law states that the absorbance equals (extinction coefficient)*(light path)*(concentration) 2 - log % Transmission natural log of the concentration times the light path length (extinction coefficient)*(light path)*(concentration) and 2 - log % Transmission (extinction coefficient)*(light path)*(concentration) and natural log of the concentration times the light path length
4. % Transmission = %T = 100*(I/Io), where Io equals light reaching the photocell light striking the cuvette 1% of the incidence light
5. Select the single best answer. The blank for a spectrophotometer should be composed of water uninoculated medium empty cuvette liquid as defined in the experiment protocol
6. The purpose of a blank is to cancel readings due to the color of the reagents to account for imperfections in the cuvette to account for imperfections in the cuvette and to cancel readings due to the absorbance of the reagents
