1. Which finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer? little finger of the dominant hand (hand holding the loop) little finger of the non-dominant hand (hand holding the sample) forefinger of the dominant hand (hand holding the loop) thumb and forefinger of the non-dominant hand (hand holding the sample)
2. The bacteriology loop should be sterilized in what part of the Bunsen burner flame? in the bright blue cone just above the bright blue cone above the flame at the base of the flame
3. The loop should be held straight up and down at a very slight angle at a fairly steep angle parallel to the work bench
