1. When transferring liquid using a pipette, the tip of the pipette should be in the center of the test tube should touch the side of the test tube never touch the side of the test tube remain in the air above the tube and never enter the tube.
2. When transferring liquid aseptically from one tube to another, you should remove the cap of the tube with the little finger of the hand holding the loop remove the cap of the tube with the little finger of the hand holding the tube place the cap on the bench top with the inside up place the cap on the bench top with the inside down
