Aseptic Techniques: Pipette Transfer
- Tilt a can of pipettes so that the tops of the pipettes are at the top of the can.
- Set the can on the laboratory bench so that the top of the can extends off the edge of the bench.
- Remove the lid from the can and set the lid on the countertop.
- Without removing it from the can, pick up a pipette with your non-dominant hand. Use your dominant hand to place a pipette aid on the pipette.
- Slide the pipette from the can, lifting it to avoid dragging the bottom of the pipette across the contaminated surfaces of the other pipettes.
- Use the little finger of the hand holding the pipette to remove the lid from the container containing your sample.
- Touch the tip of the pipette to the side of the container.
- Measure sample into the container.
- Place the pipette into a discard container and remove the pipette aid.
