1. When heat fixing a bacterial smear, you should heat the slide for 30 seconds heat the slide for 1 minute heat the slide for 2 minutes pass the slide briefly through the flame 2 or 3 times pass the slide through the flame 5-6 times
2. When making a smear from agar, you should first place a drop of water on the slide heat fix the slide to just short of red hot use as much culture as possible heat fix the slide while it is still wet
